CRISPR-mediated direct mutation of cancer genes in the mouse liver

Wen Xue, Sidi Chen, Hao Yin, Tuomas Tammela, Thales Papagiannakopoulos, Nikhil S. Joshi, Wenxin Cai, Gillian Yang, Roderick Bronson, Denise G. Crowley, Feng Zhang, Daniel G. Anderson, Phillip A. Sharp and Tyler Jacks ()
Additional contact information
Wen Xue: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Sidi Chen: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Hao Yin: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Tuomas Tammela: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Thales Papagiannakopoulos: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Nikhil S. Joshi: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Wenxin Cai: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Gillian Yang: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Roderick Bronson: Tufts University and Harvard Medical School
Denise G. Crowley: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Feng Zhang: Broad Institute of Massachusetts Institute of Technology and Harvard
Daniel G. Anderson: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Phillip A. Sharp: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Tyler Jacks: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology

Nature, 2014, vol. 514, issue 7522, 380-384

Abstract: CRISPR plasmids targeting Pten and p53, alone and in combination, are delivered by hydrodynamic injection to the liver; the CRISPR-mediated mutations phenocopy the effects of deletions using Cre–LoxP technology, allowing the direct mutation of tumour suppressor genes and oncogenes in the liver using the CRISPR/Cas system, which presents a new approach for rapid development of liver cancer models and functional genomics.

Date: 2014
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DOI: 10.1038/nature13589

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