Multiplex single-molecule interaction profiling of DNA-barcoded proteins

Liangcai Gu (), Chao Li, John Aach, David E. Hill, Marc Vidal and George M. Church ()
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Liangcai Gu: Harvard Medical School
Chao Li: Wyss Institute for Biologically Inspired Engineering, Harvard University
John Aach: Harvard Medical School
David E. Hill: Harvard Medical School
Marc Vidal: Harvard Medical School
George M. Church: Harvard Medical School

Nature, 2014, vol. 515, issue 7528, 554-557

Abstract: Single-molecular-interaction-sequencing involves attaching DNA barcodes to proteins, assaying these barcoded proteins en masse in an aqueous solution, followed by immobilization in a polyacrylamide film and amplifying and analysing the barcoding DNAs—the method allows for precise protein quantification and simultaneous interrogation of molecular binding affinity and specificity.

Date: 2014
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DOI: 10.1038/nature13761

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