Programmable RNA recognition and cleavage by CRISPR/Cas9

Mitchell R. O’Connell, Benjamin L. Oakes, Samuel H. Sternberg, Alexandra East-Seletsky, Matias Kaplan and Jennifer A. Doudna ()
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Mitchell R. O’Connell: University of California
Benjamin L. Oakes: University of California
Samuel H. Sternberg: University of California
Alexandra East-Seletsky: University of California
Matias Kaplan: Howard Hughes Medical Institute, University of California
Jennifer A. Doudna: University of California

Nature, 2014, vol. 516, issue 7530, 263-266

Abstract: In the presence of a short DNA oligonucleotide containing a protospacer adjacent motif, a guide-RNA-programmed Cas9 is able to specifically bind and/or cleave single-stranded RNA—this system can be used to isolate specific endogenous RNA transcripts from a cell lysate without any tag or modification.

Date: 2014
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DOI: 10.1038/nature13769

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