An alternative pluripotent state confers interspecies chimaeric competency

Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N. Krause, Joseph R. Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro Guillen, Josep M. Campistol, Concepcion Rodriguez Esteban, Pablo J. Ross, Alan Saghatelian, Bing Ren, Joseph R. Ecker and Juan Carlos Izpisua Belmonte ()
Additional contact information
Jun Wu: The Salk Institute for Biological Studies, Gene Expression Laboratory
Daiji Okamura: The Salk Institute for Biological Studies, Gene Expression Laboratory
Mo Li: The Salk Institute for Biological Studies, Gene Expression Laboratory
Keiichiro Suzuki: The Salk Institute for Biological Studies, Gene Expression Laboratory
Chongyuan Luo: Howard Hughes Medical Institute, The Salk Institute for Biological Studies
Li Ma: The Salk Institute for Biological Studies, Gene Expression Laboratory
Yupeng He: The Salk Institute for Biological Studies, Genomic Analysis Laboratory
Zhongwei Li: The Salk Institute for Biological Studies, Gene Expression Laboratory
Chris Benner: The Salk Institute for Biological Studies, Integrated Genomics
Isao Tamura: The Salk Institute for Biological Studies, Gene Expression Laboratory
Marie N. Krause: The Salk Institute for Biological Studies, Gene Expression Laboratory
Joseph R. Nery: The Salk Institute for Biological Studies, Genomic Analysis Laboratory
Tingting Du: Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine
Zhuzhu Zhang: The Salk Institute for Biological Studies, Genomic Analysis Laboratory
Tomoaki Hishida: The Salk Institute for Biological Studies, Gene Expression Laboratory
Yuta Takahashi: The Salk Institute for Biological Studies, Gene Expression Laboratory
Emi Aizawa: The Salk Institute for Biological Studies, Gene Expression Laboratory
Na Young Kim: The Salk Institute for Biological Studies, Gene Expression Laboratory
Jeronimo Lajara: Grado en Medicina, Universidad Católica, San Antonio de Murcia
Pedro Guillen: Grado en Medicina, Universidad Católica, San Antonio de Murcia
Josep M. Campistol: Hospital Clinic of Barcelona
Concepcion Rodriguez Esteban: The Salk Institute for Biological Studies, Gene Expression Laboratory
Pablo J. Ross: University of California, Davis
Alan Saghatelian: The Salk Institute for Biological Studies, Peptide Biology Laboratory
Bing Ren: Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine
Joseph R. Ecker: Howard Hughes Medical Institute, The Salk Institute for Biological Studies
Juan Carlos Izpisua Belmonte: The Salk Institute for Biological Studies, Gene Expression Laboratory

Nature, 2015, vol. 521, issue 7552, 316-321

Abstract: Abstract Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.

Date: 2015
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DOI: 10.1038/nature14413

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