Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai, Ved V. Topkar, Nhu T. Nguyen, Zongli Zheng, Andrew P. W. Gonzales, Zhuyun Li, Randall T. Peterson, Jing-Ruey Joanna Yeh, Martin J. Aryee and J. Keith Joung ()
Additional contact information
Benjamin P. Kleinstiver: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Michelle S. Prew: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Shengdar Q. Tsai: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Ved V. Topkar: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Nhu T. Nguyen: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Zongli Zheng: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
Andrew P. W. Gonzales: Cardiovascular Research Center, Massachusetts General Hospital
Zhuyun Li: Cardiovascular Research Center, Massachusetts General Hospital
Randall T. Peterson: Cardiovascular Research Center, Massachusetts General Hospital
Jing-Ruey Joanna Yeh: Cardiovascular Research Center, Massachusetts General Hospital
Martin J. Aryee: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital
J. Keith Joung: Molecular Pathology Unit & Center for Cancer Research, Massachusetts General Hospital

Nature, 2015, vol. 523, issue 7561, 481-485

Abstract: CRISPR-Cas9 nucleases are widely used for genome editing, but the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM); here the commonly used Streptococcus pyogenes Cas9 (SpCas9) is modified to recognize alternative PAM sequences, enabling robust editing of endogenous gene sites in zebrafish and human cells not currently targetable by wild-type SpCas9.

Date: 2015
References: Add references at CitEc
Citations: View citations in EconPapers (21)

Downloads: (external link)
https://www.nature.com/articles/nature14592 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:523:y:2015:i:7561:d:10.1038_nature14592

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature14592

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().