Panorama of ancient metazoan macromolecular complexes

Cuihong Wan, Blake Borgeson, Sadhna Phanse, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong, Olga Kagan, Julian Kwan, Alexandr Bezginov, Kyle Chessman, Swati Pal, Graham Cromar, Ophelia Papoulas, Zuyao Ni, Daniel R. Boutz, Snejana Stoilova, Pierre C. Havugimana, Xinghua Guo, Ramy H. Malty, Mihail Sarov, Jack Greenblatt, Mohan Babu, W. Brent Derry, Elisabeth R. Tillier, John B. Wallingford, John Parkinson, Edward M. Marcotte () and Andrew Emili ()
Additional contact information
Cuihong Wan: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Blake Borgeson: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Sadhna Phanse: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Fan Tu: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Kevin Drew: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Greg Clark: Department of Medical Biophysics
Xuejian Xiong: University of Toronto
Olga Kagan: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Julian Kwan: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Alexandr Bezginov: Department of Medical Biophysics
Kyle Chessman: University of Toronto
Swati Pal: Hospital for Sick Children
Graham Cromar: University of Toronto
Ophelia Papoulas: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Zuyao Ni: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Daniel R. Boutz: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Snejana Stoilova: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Pierre C. Havugimana: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Xinghua Guo: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Ramy H. Malty: University of Regina
Mihail Sarov: Max Planck Institute of Molecular Cell Biology and Genetics
Jack Greenblatt: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto
Mohan Babu: University of Regina
W. Brent Derry: University of Toronto
Elisabeth R. Tillier: Department of Medical Biophysics
John B. Wallingford: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
John Parkinson: University of Toronto
Edward M. Marcotte: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin
Andrew Emili: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto

Nature, 2015, vol. 525, issue 7569, 339-344

Abstract: Abstract Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, here we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we generated a draft conservation map consisting of more than one million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering reveals a spectrum of conservation, ranging from ancient eukaryotic assemblies that have probably served cellular housekeeping roles for at least one billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, affinity purification and functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic importance and adaptive value to animal cell systems.

Date: 2015
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DOI: 10.1038/nature14877

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