Structural basis for promiscuous PAM recognition in type I–E Cascade from E. coli

Robert P. Hayes, Yibei Xiao, Fran Ding, Paul B. G. van Erp, Kanagalaghatta Rajashankar, Scott Bailey, Blake Wiedenheft and Ailong Ke ()
Additional contact information
Robert P. Hayes: Cornell University
Yibei Xiao: Cornell University
Fran Ding: Cornell University
Paul B. G. van Erp: Montana State University
Kanagalaghatta Rajashankar: Cornell University, NE-CAT, Advanced Photon Source, Argonne National Laboratory
Scott Bailey: Johns Hopkins University, Bloomberg School of Public Health
Blake Wiedenheft: Montana State University
Ailong Ke: Cornell University

Nature, 2016, vol. 530, issue 7591, 499-503

Abstract: The structure of E. coli Cascade bound to foreign target DNA is presented, revealing the basis of the relaxed Cascade PAM recognition specificity, which results from its interaction with the minor groove, and demonstrating how a wedge in Cascade forces the directional pairing of the target strand with CRISPR RNA while stabilizing the non-target displaced strand.

Date: 2016
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DOI: 10.1038/nature16995

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