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The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

Ines Fonfara, Hagen Richter, Majda Bratovič, Anaïs Le Rhun and Emmanuelle Charpentier ()
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Ines Fonfara: The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University
Hagen Richter: Helmholtz Centre for Infection Research
Majda Bratovič: Helmholtz Centre for Infection Research
Anaïs Le Rhun: The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University
Emmanuelle Charpentier: The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University

Nature, 2016, vol. 532, issue 7600, 517-521

Abstract: The CRISPR-associated protein Cpf1 from Francisella novicida is a novel enzyme with specific, dual-endoribonuclease–endonuclease activities in precursor crRNA processing and crRNA-programmable cleavage of target DNA.

Date: 2016
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DOI: 10.1038/nature17945

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