Molecular basis of APC/C regulation by the spindle assembly checkpoint

Claudio Alfieri, Leifu Chang, Ziguo Zhang, Jing Yang, Sarah Maslen, Mark Skehel and David Barford ()
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Claudio Alfieri: MRC Laboratory of Molecular Biology, Francis Crick Avenue
Leifu Chang: MRC Laboratory of Molecular Biology, Francis Crick Avenue
Ziguo Zhang: MRC Laboratory of Molecular Biology, Francis Crick Avenue
Jing Yang: MRC Laboratory of Molecular Biology, Francis Crick Avenue
Sarah Maslen: MRC Laboratory of Molecular Biology, Francis Crick Avenue
Mark Skehel: MRC Laboratory of Molecular Biology, Francis Crick Avenue
David Barford: MRC Laboratory of Molecular Biology, Francis Crick Avenue

Nature, 2016, vol. 536, issue 7617, 431-436

Abstract: Abstract In the dividing eukaryotic cell, the spindle assembly checkpoint (SAC) ensures that each daughter cell inherits an identical set of chromosomes. The SAC coordinates the correct attachment of sister chromatid kinetochores to the mitotic spindle with activation of the anaphase-promoting complex (APC/C), the E3 ubiquitin ligase responsible for initiating chromosome separation. In response to unattached kinetochores, the SAC generates the mitotic checkpoint complex (MCC), which inhibits the APC/C and delays chromosome segregation. By cryo-electron microscopy, here we determine the near-atomic resolution structure of a human APC/C–MCC complex (APC/CMCC). Degron-like sequences of the MCC subunit BubR1 block degron recognition sites on Cdc20, the APC/C coactivator subunit responsible for substrate interactions. BubR1 also obstructs binding of the initiating E2 enzyme UbcH10 to repress APC/C ubiquitination activity. Conformational variability of the complex enables UbcH10 association, and structural analysis shows how the Cdc20 subunit intrinsic to the MCC (Cdc20MCC) is ubiquitinated, a process that results in APC/C reactivation when the SAC is silenced.

Date: 2016
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DOI: 10.1038/nature19083

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