In vitro and ex vivo strategies for intracellular delivery

Martin P. Stewart, Armon Sharei, Xiaoyun Ding, Gaurav Sahay, Robert Langer () and Klavs F. Jensen ()
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Martin P. Stewart: Massachusetts Institute of Technology
Armon Sharei: Massachusetts Institute of Technology
Xiaoyun Ding: Massachusetts Institute of Technology
Gaurav Sahay: Collaborative Life Science Building, College of Pharmacy, Oregon State University
Robert Langer: Massachusetts Institute of Technology
Klavs F. Jensen: Massachusetts Institute of Technology

Nature, 2016, vol. 538, issue 7624, 183-192

Abstract: Abstract Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.

Date: 2016
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DOI: 10.1038/nature19764

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