Integrative structure and functional anatomy of a nuclear pore complex

Seung Joong Kim, Javier Fernandez-Martinez, Ilona Nudelman, Yi Shi, Wenzhu Zhang, Barak Raveh, Thurston Herricks, Brian D. Slaughter, Joanna A. Hogan, Paula Upla, Ilan E. Chemmama, Riccardo Pellarin, Ignacia Echeverria, Manjunatha Shivaraju, Azraa S. Chaudhury, Junjie Wang, Rosemary Williams, Jay R. Unruh, Charles H. Greenberg, Erica Y. Jacobs, Zhiheng Yu, M. Jason de la Cruz, Roxana Mironska, David L. Stokes, John D. Aitchison, Martin F. Jarrold, Jennifer L. Gerton, Steven J. Ludtke (), Christopher W. Akey (), Brian T. Chait (), Andrej Sali () and Michael P. Rout ()
Additional contact information
Seung Joong Kim: University of California
Javier Fernandez-Martinez: Laboratory of Cellular and Structural Biology, The Rockefeller University
Ilona Nudelman: Laboratory of Cellular and Structural Biology, The Rockefeller University
Yi Shi: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Wenzhu Zhang: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Barak Raveh: University of California
Thurston Herricks: Institute for Systems Biology
Brian D. Slaughter: Stowers Institute for Medical Research
Joanna A. Hogan: Indiana University, Bloomington
Paula Upla: New York University School of Medicine
Ilan E. Chemmama: University of California
Riccardo Pellarin: University of California
Ignacia Echeverria: University of California
Manjunatha Shivaraju: Stowers Institute for Medical Research
Azraa S. Chaudhury: Laboratory of Cellular and Structural Biology, The Rockefeller University
Junjie Wang: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Rosemary Williams: Laboratory of Cellular and Structural Biology, The Rockefeller University
Jay R. Unruh: Stowers Institute for Medical Research
Charles H. Greenberg: University of California
Erica Y. Jacobs: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Zhiheng Yu: Janelia Research Campus, Howard Hughes Medical Institute
M. Jason de la Cruz: Janelia Research Campus, Howard Hughes Medical Institute
Roxana Mironska: Laboratory of Cellular and Structural Biology, The Rockefeller University
David L. Stokes: New York University School of Medicine
John D. Aitchison: Institute for Systems Biology
Martin F. Jarrold: Indiana University, Bloomington
Jennifer L. Gerton: Stowers Institute for Medical Research
Steven J. Ludtke: Baylor College of Medicine
Christopher W. Akey: Boston University School of Medicine
Brian T. Chait: Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University
Andrej Sali: University of California
Michael P. Rout: Laboratory of Cellular and Structural Biology, The Rockefeller University

Nature, 2018, vol. 555, issue 7697, 475-482

Abstract: Abstract Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

Date: 2018
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DOI: 10.1038/nature26003

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