Malaria parasite translocon structure and mechanism of effector export

Chi-Min Ho, Josh R. Beck, Mason Lai, Yanxiang Cui, Daniel E. Goldberg, Pascal F. Egea () and Z. Hong Zhou ()
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Chi-Min Ho: The Molecular Biology Institute, University of California
Josh R. Beck: Washington University School of Medicine
Mason Lai: Immunology & Molecular Genetics, University of California
Yanxiang Cui: University of California
Daniel E. Goldberg: Washington University School of Medicine
Pascal F. Egea: The Molecular Biology Institute, University of California
Z. Hong Zhou: The Molecular Biology Institute, University of California

Nature, 2018, vol. 561, issue 7721, 70-75

Abstract: Abstract The putative Plasmodium translocon of exported proteins (PTEX) is essential for transport of malarial effector proteins across a parasite-encasing vacuolar membrane into host erythrocytes, but the mechanism of this process remains unknown. Here we show that PTEX is a bona fide translocon by determining structures of the PTEX core complex at near-atomic resolution using cryo-electron microscopy. We isolated the endogenous PTEX core complex containing EXP2, PTEX150 and HSP101 from Plasmodium falciparum in the ‘engaged’ and ‘resetting’ states of endogenous cargo translocation using epitope tags inserted using the CRISPR–Cas9 system. In the structures, EXP2 and PTEX150 interdigitate to form a static, funnel-shaped pseudo-seven-fold-symmetric protein-conducting channel spanning the vacuolar membrane. The spiral-shaped AAA+ HSP101 hexamer is tethered above this funnel, and undergoes pronounced compaction that allows three of six tyrosine-bearing pore loops lining the HSP101 channel to dissociate from the cargo, resetting the translocon for the next threading cycle. Our work reveals the mechanism of P. falciparum effector export, and will inform structure-based design of drugs targeting this unique translocon.

Keywords: Translocon; Falciparum; Pore Loop; Endogenous Cargo; Strand Assembly (search for similar items in EconPapers)
Date: 2018
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DOI: 10.1038/s41586-018-0469-4

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