POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Anaxi Houbaert, Cheng Zhang, Manish Tiwari, Kun Wang, Alberto Marcos Serrano, Daniel V. Savatin, Mounashree J. Urs, Miroslava K. Zhiponova, Gustavo E. Gudesblat, Isabelle Vanhoutte, Dominique Eeckhout, Sjef Boeren, Mansour Karimi, Camilla Betti, Thomas Jacobs, Carmen Fenoll, Montaña Mena, Sacco Vries, Geert Jaeger and Eugenia Russinova ()
Additional contact information
Anaxi Houbaert: Ghent University
Cheng Zhang: Ghent University
Manish Tiwari: Ghent University
Kun Wang: Ghent University
Alberto Marcos Serrano: Universidad de Castilla-la Mancha
Daniel V. Savatin: Ghent University
Mounashree J. Urs: Ghent University
Miroslava K. Zhiponova: Ghent University
Gustavo E. Gudesblat: Ghent University
Isabelle Vanhoutte: Ghent University
Dominique Eeckhout: Ghent University
Sjef Boeren: Wageningen University
Mansour Karimi: Ghent University
Camilla Betti: Ghent University
Thomas Jacobs: Ghent University
Carmen Fenoll: Universidad de Castilla-la Mancha
Montaña Mena: Universidad de Castilla-la Mancha
Sacco Vries: Wageningen University
Geert Jaeger: Ghent University
Eugenia Russinova: Ghent University

Nature, 2018, vol. 563, issue 7732, 574-578

Abstract: Abstract Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.

Keywords: Stomatal Lineage; Speechless (SPCH); Pavement Cells; Meristemoids; Vector pK7FWG2 (search for similar items in EconPapers)
Date: 2018
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DOI: 10.1038/s41586-018-0714-x

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