The emergent landscape of the mouse gut endoderm at single-cell resolution

Sonja Nowotschin, Manu Setty, Ying-Yi Kuo, Vincent Liu, Vidur Garg, Roshan Sharma, Claire S. Simon, Nestor Saiz, Rui Gardner, Stéphane C. Boutet, Deanna M. Church, Pamela A. Hoodless, Anna-Katerina Hadjantonakis () and Dana Pe’er ()
Additional contact information
Sonja Nowotschin: Memorial Sloan Kettering Cancer Center
Manu Setty: Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
Ying-Yi Kuo: Memorial Sloan Kettering Cancer Center
Vincent Liu: Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
Vidur Garg: Memorial Sloan Kettering Cancer Center
Roshan Sharma: Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
Claire S. Simon: Memorial Sloan Kettering Cancer Center
Nestor Saiz: Memorial Sloan Kettering Cancer Center
Rui Gardner: Memorial Sloan Kettering Cancer Center
Stéphane C. Boutet: 10x Genomics
Deanna M. Church: 10x Genomics
Pamela A. Hoodless: Terry Fox Laboratory, BC Cancer
Anna-Katerina Hadjantonakis: Memorial Sloan Kettering Cancer Center
Dana Pe’er: Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center

Nature, 2019, vol. 569, issue 7756, 361-367

Abstract: Abstract Here we delineate the ontogeny of the mammalian endoderm by generating 112,217 single-cell transcriptomes, which represent all endoderm populations within the mouse embryo until midgestation. We use graph-based approaches to model differentiating cells, which provides a spatio-temporal characterization of developmental trajectories and defines the transcriptional architecture that accompanies the emergence of the first (primitive or extra-embryonic) endodermal population and its sister pluripotent (embryonic) epiblast lineage. We uncover a relationship between descendants of these two lineages, in which epiblast cells differentiate into endoderm at two distinct time points—before and during gastrulation. Trajectories of endoderm cells were mapped as they acquired embryonic versus extra-embryonic fates and as they spatially converged within the nascent gut endoderm, which revealed these cells to be globally similar but retain aspects of their lineage history. We observed the regionalized identity of cells along the anterior–posterior axis of the emergent gut tube, which reflects their embryonic or extra-embryonic origin, and the coordinated patterning of these cells into organ-specific territories.

Date: 2019
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DOI: 10.1038/s41586-019-1127-1

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