Single-cell lineage tracing unveils a role for TCF15 in haematopoiesis

Alejo E. Rodriguez-Fraticelli, Caleb Weinreb, Shou-Wen Wang, Rosa P. Migueles, Maja Jankovic, Marc Usart, Allon M. Klein, Sally Lowell and Fernando D. Camargo ()
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Alejo E. Rodriguez-Fraticelli: Harvard University
Caleb Weinreb: Department of Systems Biology
Shou-Wen Wang: Department of Systems Biology
Rosa P. Migueles: The University of Edinburgh
Maja Jankovic: Harvard University
Marc Usart: Harvard University
Allon M. Klein: Department of Systems Biology
Sally Lowell: The University of Edinburgh
Fernando D. Camargo: Harvard University

Nature, 2020, vol. 583, issue 7817, 585-589

Abstract: Abstract Bone marrow transplantation therapy relies on the life-long regenerative capacity of haematopoietic stem cells (HSCs)1,2. HSCs present a complex variety of regenerative behaviours at the clonal level, but the mechanisms underlying this diversity are still undetermined3–11. Recent advances in single-cell RNA sequencing have revealed transcriptional differences among HSCs, providing a possible explanation for their functional heterogeneity12–17. However, the destructive nature of sequencing assays prevents simultaneous observation of stem cell state and function. To solve this challenge, we implemented expressible lentiviral barcoding, which enabled simultaneous analysis of lineages and transcriptomes from single adult HSCs and their clonal trajectories during long-term bone marrow reconstitution. Analysis of differential gene expression between clones with distinct behaviour revealed an intrinsic molecular signature that characterizes functional long-term repopulating HSCs. Probing this signature through in vivo CRISPR screening, we found the transcription factor TCF15 to be required and sufficient to drive HSC quiescence and long-term self-renewal. In situ, Tcf15 expression labels the most primitive subset of true multipotent HSCs. In conclusion, our work elucidates clone-intrinsic molecular programmes associated with functional stem cell heterogeneity and identifies a mechanism for the maintenance of the self-renewing HSC state.

Date: 2020
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DOI: 10.1038/s41586-020-2503-6

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