Human neutralizing antibodies elicited by SARS-CoV-2 infection

Bin Ju, Qi Zhang, Jiwan Ge, Ruoke Wang, Jing Sun, Xiangyang Ge, Jiazhen Yu, Sisi Shan, Bing Zhou, Shuo Song, Xian Tang, Jinfang Yu, Jun Lan, Jing Yuan, Haiyan Wang, Juanjuan Zhao, Shuye Zhang, Youchun Wang, Xuanling Shi, Lei Liu, Jincun Zhao, Xinquan Wang (), Zheng Zhang () and Linqi Zhang ()
Additional contact information
Bin Ju: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Qi Zhang: Tsinghua University
Jiwan Ge: Tsinghua University
Ruoke Wang: Tsinghua University
Jing Sun: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University
Xiangyang Ge: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Jiazhen Yu: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Sisi Shan: Tsinghua University
Bing Zhou: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Shuo Song: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Xian Tang: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Jinfang Yu: Tsinghua University
Jun Lan: Tsinghua University
Jing Yuan: Shenzhen Third People’s Hospital
Haiyan Wang: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Juanjuan Zhao: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Shuye Zhang: Fudan University
Youchun Wang: Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, National Institutes for Food and Drug Control
Xuanling Shi: Tsinghua University
Lei Liu: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Jincun Zhao: State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University
Xinquan Wang: Tsinghua University
Zheng Zhang: Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital
Linqi Zhang: Tsinghua University

Nature, 2020, vol. 584, issue 7819, 115-119

Abstract: Abstract The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a global health emergency that is in urgent need of intervention1–3. The entry of SARS-CoV-2 into its target cells depends on binding between the receptor-binding domain (RBD) of the viral spike protein and its cellular receptor, angiotensin-converting enzyme 2 (ACE2)2,4–6. Here we report the isolation and characterization of 206 RBD-specific monoclonal antibodies derived from single B cells from 8 individuals infected with SARS-CoV-2. We identified antibodies that potently neutralize SARS-CoV-2; this activity correlates with competition with ACE2 for binding to RBD. Unexpectedly, the anti-SARS-CoV-2 antibodies and the infected plasma did not cross-react with the RBDs of SARS-CoV or Middle East respiratory syndrome-related coronavirus (MERS-CoV), although there was substantial plasma cross-reactivity to their trimeric spike proteins. Analysis of the crystal structure of RBD-bound antibody revealed that steric hindrance inhibits viral engagement with ACE2, thereby blocking viral entry. These findings suggest that anti-RBD antibodies are largely viral-species-specific inhibitors. The antibodies identified here may be candidates for development of clinical interventions against SARS-CoV-2.

Date: 2020
References: Add references at CitEc
Citations: View citations in EconPapers (11)

Downloads: (external link)
https://www.nature.com/articles/s41586-020-2380-z Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:584:y:2020:i:7819:d:10.1038_s41586-020-2380-z

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/s41586-020-2380-z

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().