Structural basis of CXC chemokine receptor 2 activation and signalling

Kaiwen Liu, Lijie Wu, Shuguang Yuan, Meng Wu, Yueming Xu, Qianqian Sun, Shu Li, Suwen Zhao, Tian Hua () and Zhi-Jie Liu ()
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Kaiwen Liu: ShanghaiTech University
Lijie Wu: ShanghaiTech University
Shuguang Yuan: Chinese Academy of Sciences
Meng Wu: ShanghaiTech University
Yueming Xu: ShanghaiTech University
Qianqian Sun: ShanghaiTech University
Shu Li: Chinese Academy of Sciences
Suwen Zhao: ShanghaiTech University
Tian Hua: ShanghaiTech University
Zhi-Jie Liu: ShanghaiTech University

Nature, 2020, vol. 585, issue 7823, 135-140

Abstract: Abstract Chemokines and their receptors mediate cell migration, which influences multiple fundamental biological processes and disease conditions such as inflammation and cancer1. Although ample effort has been invested into the structural investigation of the chemokine receptors and receptor–chemokine recognition2–4, less is known about endogenous chemokine-induced receptor activation and G-protein coupling. Here we present the cryo-electron microscopy structures of interleukin-8 (IL-8, also known as CXCL8)-activated human CXC chemokine receptor 2 (CXCR2) in complex with Gi protein, along with a crystal structure of CXCR2 bound to a designed allosteric antagonist. Our results reveal a unique shallow mode of binding between CXCL8 and CXCR2, and also show the interactions between CXCR2 and Gi protein. Further structural analysis of the inactive and active states of CXCR2 reveals a distinct activation process and the competitive small-molecule antagonism of chemokine receptors. In addition, our results provide insights into how a G-protein-coupled receptor is activated by an endogenous protein molecule, which will assist in the rational development of therapeutics that target the chemokine system for better pharmacological profiles.

Date: 2020
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DOI: 10.1038/s41586-020-2492-5

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