The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

Kathrin Thor, Shushu Jiang, Erwan Michard, Jeoffrey George, Sönke Scherzer, Shouguang Huang, Julian Dindas, Paul Derbyshire, Nuno Leitão, Thomas A. DeFalco, Philipp Köster, Kerri Hunter, Sachie Kimura, Julien Gronnier, Lena Stransfeld, Yasuhiro Kadota, Christoph A. Bücherl, Myriam Charpentier, Michael Wrzaczek, Daniel MacLean, Giles E. D. Oldroyd, Frank L. H. Menke, M. Rob G. Roelfsema, Rainer Hedrich, José Feijó and Cyril Zipfel ()
Additional contact information
Kathrin Thor: University of East Anglia, Norwich Research Park
Shushu Jiang: University of East Anglia, Norwich Research Park
Erwan Michard: University of Maryland, Department of Cell Biology and Molecular Genetics
Jeoffrey George: University of East Anglia, Norwich Research Park
Sönke Scherzer: University of Würzburg
Shouguang Huang: University of Würzburg
Julian Dindas: University of Zurich
Paul Derbyshire: University of East Anglia, Norwich Research Park
Nuno Leitão: John Innes Centre, Norwich Research Park
Thomas A. DeFalco: University of East Anglia, Norwich Research Park
Philipp Köster: University of Zurich
Kerri Hunter: University of Helsinki
Sachie Kimura: University of Helsinki
Julien Gronnier: University of East Anglia, Norwich Research Park
Lena Stransfeld: University of East Anglia, Norwich Research Park
Yasuhiro Kadota: University of East Anglia, Norwich Research Park
Christoph A. Bücherl: University of East Anglia, Norwich Research Park
Myriam Charpentier: John Innes Centre, Norwich Research Park
Michael Wrzaczek: University of Helsinki
Daniel MacLean: University of East Anglia, Norwich Research Park
Giles E. D. Oldroyd: John Innes Centre, Norwich Research Park
Frank L. H. Menke: University of East Anglia, Norwich Research Park
M. Rob G. Roelfsema: University of Würzburg
Rainer Hedrich: University of Würzburg
José Feijó: University of Maryland, Department of Cell Biology and Molecular Genetics
Cyril Zipfel: University of East Anglia, Norwich Research Park

Nature, 2020, vol. 585, issue 7826, 569-573

Abstract: Abstract Perception of biotic and abiotic stresses often leads to stomatal closure in plants1,2. Rapid influx of calcium ions (Ca2+) across the plasma membrane has an important role in this response, but the identity of the Ca2+ channels involved has remained elusive3,4. Here we report that the Arabidopsis thaliana Ca2+-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid—a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca2+ channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca2+ influx mechanisms in response to different stresses.

Date: 2020
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DOI: 10.1038/s41586-020-2702-1

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