A proximity-dependent biotinylation map of a human cell

Christopher D. Go, James D. R. Knight, Archita Rajasekharan, Bhavisha Rathod, Geoffrey G. Hesketh, Kento T. Abe, Ji-Young Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y. Zhu, Evelyn Popiel, Jean-Philippe Lambert, Étienne Coyaud, Sally W. T. Cheung, Dushyandi Rajendran, Cassandra J. Wong, Hana Antonicka, Laurence Pelletier, Alexander F. Palazzo, Eric A. Shoubridge, Brian Raught and Anne-Claude Gingras ()
Additional contact information
Christopher D. Go: Mount Sinai Hospital, Sinai Health System
James D. R. Knight: Mount Sinai Hospital, Sinai Health System
Archita Rajasekharan: McGill University
Bhavisha Rathod: Mount Sinai Hospital, Sinai Health System
Geoffrey G. Hesketh: Mount Sinai Hospital, Sinai Health System
Kento T. Abe: Mount Sinai Hospital, Sinai Health System
Ji-Young Youn: Mount Sinai Hospital, Sinai Health System
Payman Samavarchi-Tehrani: Mount Sinai Hospital, Sinai Health System
Hui Zhang: University of Toronto
Lucie Y. Zhu: University of Toronto
Evelyn Popiel: University of Toronto
Jean-Philippe Lambert: Mount Sinai Hospital, Sinai Health System
Étienne Coyaud: University Health Network
Sally W. T. Cheung: Mount Sinai Hospital, Sinai Health System
Dushyandi Rajendran: Mount Sinai Hospital, Sinai Health System
Cassandra J. Wong: Mount Sinai Hospital, Sinai Health System
Hana Antonicka: McGill University
Laurence Pelletier: Mount Sinai Hospital, Sinai Health System
Alexander F. Palazzo: University of Toronto
Eric A. Shoubridge: McGill University
Brian Raught: University Health Network
Anne-Claude Gingras: Mount Sinai Hospital, Sinai Health System

Nature, 2021, vol. 595, issue 7865, 120-124

Abstract: Abstract Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2–4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells5–7. Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of 4,145 unique proteins in HEK293 cells. Our localization predictions exceed the specificity of previous approaches, and enabled the discovery of proteins at the interface between the mitochondrial outer membrane and the endoplasmic reticulum that are crucial for mitochondrial homeostasis. On the basis of this dataset, we created humancellmap.org as a community resource that provides online tools for localization analysis of user BioID data, and demonstrate how this resource can be used to understand BioID results better.

Date: 2021
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DOI: 10.1038/s41586-021-03592-2

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