Mechanisms of BRCA1–BARD1 nucleosome recognition and ubiquitylation

Qi Hu, Maria Victoria Botuyan, Debiao Zhao, Gaofeng Cui, Elie Mer and Georges Mer ()
Additional contact information
Qi Hu: Mayo Clinic
Maria Victoria Botuyan: Mayo Clinic
Debiao Zhao: Mayo Clinic
Gaofeng Cui: Mayo Clinic
Elie Mer: Mayo Clinic
Georges Mer: Mayo Clinic

Nature, 2021, vol. 596, issue 7872, 438-443

Abstract: Abstract The BRCA1–BARD1 tumour suppressor is an E3 ubiquitin ligase necessary for the repair of DNA double-strand breaks by homologous recombination1–10. The BRCA1–BARD1 complex localizes to damaged chromatin after DNA replication and catalyses the ubiquitylation of histone H2A and other cellular targets11–14. The molecular bases for the recruitment to double-strand breaks and target recognition of BRCA1–BARD1 remain unknown. Here we use cryo-electron microscopy to show that the ankyrin repeat and tandem BRCT domains in BARD1 adopt a compact fold and bind to nucleosomal histones, DNA and monoubiquitin attached to H2A amino-terminal K13 or K15, two signals known to be specific for double-strand breaks15,16. We further show that RING domains17 in BRCA1–BARD1 orient an E2 ubiquitin-conjugating enzyme atop the nucleosome in a dynamic conformation, primed for ubiquitin transfer to the flexible carboxy-terminal tails of H2A and variant H2AX. Our work reveals a regulatory crosstalk in which recognition of monoubiquitin by BRCA1–BARD1 at the N terminus of H2A blocks the formation of polyubiquitin chains and cooperatively promotes ubiquitylation at the C terminus of H2A. These findings elucidate the mechanisms of BRCA1–BARD1 chromatin recruitment and ubiquitylation specificity, highlight key functions of BARD1 in both processes and explain how BRCA1–BARD1 promotes homologous recombination by opposing the DNA repair protein 53BP1 in post-replicative chromatin18–22. These data provide a structural framework to evaluate BARD1 variants and help to identify mutations that drive the development of cancer.

Date: 2021
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DOI: 10.1038/s41586-021-03716-8

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