Parallelism of intestinal secretory IgA shapes functional microbial fitness

Tim Rollenske (), Sophie Burkhalter, Lukas Muerner, Stephan Gunten, Jolanta Lukasiewicz, Hedda Wardemann and Andrew J. Macpherson ()
Additional contact information
Tim Rollenske: University Clinic of Visceral Surgery and Medicine, Inselspital, University of Bern
Sophie Burkhalter: University Clinic of Visceral Surgery and Medicine, Inselspital, University of Bern
Lukas Muerner: University of Bern
Stephan Gunten: University of Bern
Jolanta Lukasiewicz: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
Hedda Wardemann: German Cancer Research Center
Andrew J. Macpherson: University Clinic of Visceral Surgery and Medicine, Inselspital, University of Bern

Nature, 2021, vol. 598, issue 7882, 657-661

Abstract: Abstract Dimeric IgA secreted across mucous membranes in response to nonpathogenic taxa of the microbiota accounts for most antibody production in mammals. Diverse binding specificities can be detected within the polyclonal mucosal IgA antibody response1–10, but limited monoclonal hybridomas have been studied to relate antigen specificity or polyreactive binding to functional effects on microbial physiology in vivo11–17. Here we use recombinant dimeric monoclonal IgAs (mIgAs) to finely map the intestinal plasma cell response to microbial colonization with a single microorganism in mice. We identify a range of antigen-specific mIgA molecules targeting defined surface and nonsurface membrane antigens. Secretion of individual dimeric mIgAs targeting different antigens in vivo showed distinct alterations in the function and metabolism of intestinal bacteria, largely through specific binding. Even in cases in which the same microbial antigen is targeted, microbial metabolic alterations differed depending on IgA epitope specificity. By contrast, bacterial surface coating generally reduced motility and limited bile acid toxicity. The overall intestinal IgA response to a single microbe therefore contains parallel components with distinct effects on microbial carbon-source uptake, bacteriophage susceptibility, motility and membrane integrity.

Date: 2021
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41586-021-03973-7 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:598:y:2021:i:7882:d:10.1038_s41586-021-03973-7

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/s41586-021-03973-7

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().