Genome surveillance by HUSH-mediated silencing of intronless mobile elements

Marta Seczynska, Stuart Bloor, Sergio Martinez Cuesta and Paul J. Lehner ()
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Marta Seczynska: University of Cambridge
Stuart Bloor: University of Cambridge
Sergio Martinez Cuesta: AstraZeneca, Cambridge Biomedical Campus
Paul J. Lehner: University of Cambridge

Nature, 2022, vol. 601, issue 7893, 440-445

Abstract: Abstract All life forms defend their genome against DNA invasion. Eukaryotic cells recognize incoming DNA and limit its transcription through repressive chromatin modifications. The human silencing hub (HUSH) complex transcriptionally represses long interspersed element-1 retrotransposons (L1s) and retroviruses through histone H3 lysine 9 trimethylation (H3K9me3)1–3. How HUSH recognizes and initiates silencing of these invading genetic elements is unknown. Here we show that HUSH is able to recognize and transcriptionally repress a broad range of long, intronless transgenes. Intron insertion into HUSH-repressed transgenes counteracts repression, even in the absence of intron splicing. HUSH binds transcripts from the target locus, prior to and independent of H3K9me3 deposition, and target transcription is essential for both initiation and propagation of HUSH-mediated H3K9me3. Genomic data reveal how HUSH binds and represses a subset of endogenous intronless genes generated through retrotransposition of cellular mRNAs. Thus intronless cDNA—the hallmark of reverse transcription—provides a versatile way to distinguish invading retroelements from host genes and enables HUSH to protect the genome from ‘non-self’ DNA, despite there being no previous exposure to the invading element. Our findings reveal the existence of a transcription-dependent genome-surveillance system and explain how it provides immediate protection against newly acquired elements while avoiding inappropriate repression of host genes.

Date: 2022
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DOI: 10.1038/s41586-021-04228-1

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