 Long-primed germinal centres with enduring affinity maturation and clonal migrationJeong Hyun Lee,
Henry J. Sutton,
Christopher A. Cottrell,
Ivy Phung,
Gabriel Ozorowski,
Leigh M. Sewall,
Rebecca Nedellec,
Catherine Nakao,
Murillo Silva,
Sara T. Richey,
Jonathan L. Torres,
Wen-Hsin Lee,
Erik Georgeson,
Michael Kubitz,
Sam Hodges,
Tina-Marie Mullen,
Yumiko Adachi,
Kimberly M. Cirelli,
Amitinder Kaur,
Carolina Allers,
Marissa Fahlberg,
Brooke F. Grasperge,
Jason P. Dufour,
Faith Schiro,
Pyone P. Aye,
Oleksandr Kalyuzhniy,
Alessia Liguori,
Diane G. Carnathan,
Guido Silvestri,
Xiaoying Shen,
David C. Montefiori,
Ronald S. Veazey,
Andrew B. Ward,
Lars Hangartner,
Dennis R. Burton,
Darrell J. Irvine,
William R. Schief and
Shane Crotty ()
Nature, 2022, vol. 609, issue 7929, 998-1004 Abstract: Abstract Germinal centres are the engines of antibody evolution. Here, using human immunodeficiency virus (HIV) Env protein immunogen priming in rhesus monkeys followed by a long period without further immunization, we demonstrate germinal centre B (BGC) cells that last for at least 6 months. A 186-fold increase in BGC cells was present by week 10 compared with conventional immunization. Single-cell transcriptional profiling showed that both light- and dark-zone germinal centre states were sustained. Antibody somatic hypermutation of BGC cells continued to accumulate throughout the 29-week priming period, with evidence of selective pressure. Env-binding BGC cells were still 49-fold above baseline at 29 weeks, which suggests that they could remain active for even longer periods of time. High titres of HIV-neutralizing antibodies were generated after a single booster immunization. Fully glycosylated HIV trimer protein is a complex antigen, posing considerable immunodominance challenges for B cells1,2. Memory B cells generated under these long priming conditions had higher levels of antibody somatic hypermutation, and both memory B cells and antibodies were more likely to recognize non-immunodominant epitopes. Numerous BGC cell lineage phylogenies spanning more than the 6-month germinal centre period were identified, demonstrating continuous germinal centre activity and selection for at least 191 days with no further antigen exposure. A long-prime, slow-delivery (12 days) immunization approach holds promise for difficult vaccine targets and suggests that patience can have great value for tuning of germinal centres to maximize antibody responses. Date: 2022 Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:609:y:2022:i:7929:d:10.1038_s41586-022-05216-9 Ordering information: This journal article can be ordered from DOI: 10.1038/s41586-022-05216-9 Access Statistics for this article Nature is currently edited by Magdalena Skipper More articles in Nature from Nature |
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