Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

Linlin Qi, Mateusz Kwiatkowski, Huihuang Chen, Lukas Hoermayer, Scott Sinclair, Minxia Zou, Charo I. Genio, Martin F. Kubeš, Richard Napier, Krzysztof Jaworski and Jiří Friml ()
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Linlin Qi: Institute of Science and Technology Austria (ISTA)
Mateusz Kwiatkowski: Nicolaus Copernicus University in Toruń
Huihuang Chen: Institute of Science and Technology Austria (ISTA)
Lukas Hoermayer: Institute of Science and Technology Austria (ISTA)
Scott Sinclair: Institute of Science and Technology Austria (ISTA)
Minxia Zou: Institute of Science and Technology Austria (ISTA)
Charo I. Genio: Coventry University
Martin F. Kubeš: University of Warwick
Richard Napier: University of Warwick
Krzysztof Jaworski: Nicolaus Copernicus University in Toruń
Jiří Friml: Institute of Science and Technology Austria (ISTA)

Nature, 2022, vol. 611, issue 7934, 133-138

Abstract: Abstract The phytohormone auxin is the major coordinative signal in plant development1, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination2,3. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.

Date: 2022
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DOI: 10.1038/s41586-022-05369-7

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