Primate gastrulation and early organogenesis at single-cell resolution

Jinglei Zhai, Jing Guo, Haifeng Wan, Luqing Qi, Lizhong Liu, Zhenyu Xiao, Long Yan, Daniel A. Schmitz, Yanhong Xu, Dainan Yu, Xulun Wu, Wentao Zhao, Kunyuan Yu, Xiangxiang Jiang (), Fan Guo (), Jun Wu () and Hongmei Wang ()
Additional contact information
Jinglei Zhai: Chinese Academy of Sciences
Jing Guo: Chinese Academy of Sciences
Haifeng Wan: Chinese Academy of Sciences
Luqing Qi: Chinese Academy of Sciences
Lizhong Liu: University of Texas Southwestern Medical Center
Zhenyu Xiao: Chinese Academy of Sciences
Long Yan: Chinese Academy of Sciences
Daniel A. Schmitz: University of Texas Southwestern Medical Center
Yanhong Xu: Chinese Academy of Sciences
Dainan Yu: Chinese Academy of Sciences
Xulun Wu: Chinese Academy of Sciences
Wentao Zhao: Chinese Academy of Sciences
Kunyuan Yu: Chinese Academy of Sciences
Xiangxiang Jiang: Chinese Academy of Sciences
Fan Guo: Chinese Academy of Sciences
Jun Wu: University of Texas Southwestern Medical Center
Hongmei Wang: Chinese Academy of Sciences

Nature, 2022, vol. 612, issue 7941, 732-738

Abstract: Abstract Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due to their close evolutionary relationship with humans, nonhuman primates are often used as surrogates to understand human development but currently suffer from a lack of in vivo datasets, especially from gastrulation to early organogenesis during which the major embryonic cell types are dynamically specified. To fill this gap, we collected six Carnegie stage 8–11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth transcriptomic analyses of 56,636 single cells. Our analyses show transcriptomic features of major perigastrulation cell types, which help shed light on morphogenetic events including primitive streak development, somitogenesis, gut tube formation, neural tube patterning and neural crest differentiation in primates. In addition, comparative analyses with mouse embryos and human embryoids uncovered conserved and divergent features of perigastrulation development across species—for example, species-specific dependency on Hippo signalling during presomitic mesoderm differentiation—and provide an initial assessment of relevant stem cell models of human early organogenesis. This comprehensive single-cell transcriptome atlas not only fills the knowledge gap in the nonhuman primate research field but also serves as an invaluable resource for understanding human embryogenesis and developmental disorders.

Date: 2022
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DOI: 10.1038/s41586-022-05526-y

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