Generation of functional oocytes from male mice in vitro

Kenta Murakami, Nobuhiko Hamazaki, Norio Hamada, Go Nagamatsu, Ikuhiro Okamoto, Hiroshi Ohta, Yoshiaki Nosaka, Yukiko Ishikura, Tomoya S. Kitajima, Yuichiro Semba, Yuya Kunisaki, Fumio Arai, Koichi Akashi, Mitinori Saitou, Kiyoko Kato and Katsuhiko Hayashi ()
Additional contact information
Kenta Murakami: Kyushu University
Nobuhiko Hamazaki: Kyushu University
Norio Hamada: Kyushu University
Go Nagamatsu: Kyushu University
Ikuhiro Okamoto: Kyoto University
Hiroshi Ohta: Kyoto University
Yoshiaki Nosaka: Kyoto University
Yukiko Ishikura: Kyoto University
Tomoya S. Kitajima: RIKEN Center for Biosystems Dynamics Research
Yuichiro Semba: Kyushu University
Yuya Kunisaki: Kyushu University
Fumio Arai: Kyushu University
Koichi Akashi: Kyushu University
Mitinori Saitou: Kyoto University
Kiyoko Kato: Kyushu University
Katsuhiko Hayashi: Kyushu University

Nature, 2023, vol. 615, issue 7954, 900-906

Abstract: Abstract Sex chromosome disorders severely compromise gametogenesis in both males and females. In oogenesis, the presence of an additional Y chromosome or the loss of an X chromosome disturbs the robust production of oocytes1–5. Here we efficiently converted the XY chromosome set to XX without an additional Y chromosome in mouse pluripotent stem (PS) cells. In addition, this chromosomal alteration successfully eradicated trisomy 16, a model of Down’s syndrome, in PS cells. Artificially produced euploid XX PS cells differentiated into mature oocytes in culture with similar efficiency to native XX PS cells. Using this method, we differentiated induced pluripotent stem cells from the tail of a sexually mature male mouse into fully potent oocytes, which gave rise to offspring after fertilization. This study provides insights that could ameliorate infertility caused by sex chromosome or autosomal disorders, and opens the possibility of bipaternal reproduction.

Date: 2023
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DOI: 10.1038/s41586-023-05834-x

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