Fanzor is a eukaryotic programmable RNA-guided endonuclease

Makoto Saito, Peiyu Xu, Guilhem Faure, Samantha Maguire, Soumya Kannan, Han Altae-Tran, Sam Vo, AnAn Desimone, Rhiannon K. Macrae and Feng Zhang ()
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Makoto Saito: Broad Institute of MIT and Harvard
Peiyu Xu: Broad Institute of MIT and Harvard
Guilhem Faure: Broad Institute of MIT and Harvard
Samantha Maguire: Broad Institute of MIT and Harvard
Soumya Kannan: Broad Institute of MIT and Harvard
Han Altae-Tran: Broad Institute of MIT and Harvard
Sam Vo: Broad Institute of MIT and Harvard
AnAn Desimone: Broad Institute of MIT and Harvard
Rhiannon K. Macrae: Broad Institute of MIT and Harvard
Feng Zhang: Broad Institute of MIT and Harvard

Nature, 2023, vol. 620, issue 7974, 660-668

Abstract: Abstract RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR–Cas systems provide adaptive immunity for bacteria and archaea against foreign genetic elements. Cas effectors such as Cas9 and Cas12 perform guide-RNA-dependent DNA cleavage1. Although a few eukaryotic RNA-guided systems have been studied, including RNA interference2 and ribosomal RNA modification3, it remains unclear whether eukaryotes have RNA-guided endonucleases. Recently, a new class of prokaryotic RNA-guided systems (termed OMEGA) was reported4,5. The OMEGA effector TnpB is the putative ancestor of Cas12 and has RNA-guided endonuclease activity4,6. TnpB may also be the ancestor of the eukaryotic transposon-encoded Fanzor (Fz) proteins4,7, raising the possibility that eukaryotes are also equipped with CRISPR–Cas or OMEGA-like programmable RNA-guided endonucleases. Here we report the biochemical characterization of Fz, showing that it is an RNA-guided DNA endonuclease. We also show that Fz can be reprogrammed for human genome engineering applications. Finally, we resolve the structure of Spizellomyces punctatus Fz at 2.7 Å using cryogenic electron microscopy, showing the conservation of core regions among Fz, TnpB and Cas12, despite diverse cognate RNA structures. Our results show that Fz is a eukaryotic OMEGA system, demonstrating that RNA-guided endonucleases are present in all three domains of life.

Date: 2023
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DOI: 10.1038/s41586-023-06356-2

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