Structural basis of Integrator-dependent RNA polymerase II termination

Isaac Fianu (), Moritz Ochmann, James L. Walshe, Olexandr Dybkov, Joseph Neos Cruz, Henning Urlaub and Patrick Cramer ()
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Isaac Fianu: Max Planck Institute for Multidisciplinary Sciences
Moritz Ochmann: Max Planck Institute for Multidisciplinary Sciences
James L. Walshe: Max Planck Institute for Multidisciplinary Sciences
Olexandr Dybkov: Max Planck Institute for Multidisciplinary Sciences
Joseph Neos Cruz: Max Planck Institute for Multidisciplinary Sciences
Henning Urlaub: Max Planck Institute for Multidisciplinary Sciences
Patrick Cramer: Max Planck Institute for Multidisciplinary Sciences

Nature, 2024, vol. 629, issue 8010, 219-227

Abstract: Abstract The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal region of genes. Previous work has shed light on how Integrator binds to the paused elongation complex consisting of Pol II, the DRB sensitivity-inducing factor (DSIF) and the negative elongation factor (NELF) and how it cleaves the nascent RNA transcript1, but has not explained how Integrator removes Pol II from the DNA template. Here we present three cryo-electron microscopy structures of the complete Integrator–PP2A complex in different functional states. The structure of the pre-termination complex reveals a previously unresolved, scorpion-tail-shaped INTS10–INTS13–INTS14–INTS15 module that may use its ‘sting’ to open the DSIF DNA clamp and facilitate termination. The structure of the post-termination complex shows that the previously unresolved subunit INTS3 and associated sensor of single-stranded DNA complex (SOSS) factors prevent Pol II rebinding to Integrator after termination. The structure of the free Integrator–PP2A complex in an inactive closed conformation2 reveals that INTS6 blocks the PP2A phosphatase active site. These results lead to a model for how Integrator terminates Pol II transcription in three steps that involve major rearrangements.

Date: 2024
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DOI: 10.1038/s41586-024-07269-4

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