Structural mechanism of angiogenin activation by the ribosome

Anna B. Loveland (), Cha San Koh, Robin Ganesan, Allan Jacobson and Andrei A. Korostelev ()
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Anna B. Loveland: UMass Chan Medical School
Cha San Koh: UMass Chan Medical School
Robin Ganesan: UMass Chan Medical School
Allan Jacobson: UMass Chan Medical School
Andrei A. Korostelev: UMass Chan Medical School

Nature, 2024, vol. 630, issue 8017, 769-776

Abstract: Abstract Angiogenin, an RNase-A-family protein, promotes angiogenesis and has been implicated in cancer, neurodegenerative diseases and epigenetic inheritance1–10. After activation during cellular stress, angiogenin cleaves tRNAs at the anticodon loop, resulting in translation repression11–15. However, the catalytic activity of isolated angiogenin is very low, and the mechanisms of the enzyme activation and tRNA specificity have remained a puzzle3,16–23. Here we identify these mechanisms using biochemical assays and cryogenic electron microscopy (cryo-EM). Our study reveals that the cytosolic ribosome is the activator of angiogenin. A cryo-EM structure features angiogenin bound in the A site of the 80S ribosome. The C-terminal tail of angiogenin is rearranged by interactions with the ribosome to activate the RNase catalytic centre, making the enzyme several orders of magnitude more efficient in tRNA cleavage. Additional 80S–angiogenin structures capture how tRNA substrate is directed by the ribosome into angiogenin’s active site, demonstrating that the ribosome acts as the specificity factor. Our findings therefore suggest that angiogenin is activated by ribosomes with a vacant A site, the abundance of which increases during cellular stress24–27. These results may facilitate the development of therapeutics to treat cancer and neurodegenerative diseases.

Date: 2024
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DOI: 10.1038/s41586-024-07508-8

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