A bacterial immunity protein directly senses two disparate phage proteins

Tong Zhang, Albinas Cepauskas, Anastasiia Nadieina, Aurelien Thureau, Kyo Coppieters ‘t Wallant, Chloé Martens, Daniel C. Lim, Abel Garcia-Pino () and Michael T. Laub ()
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Tong Zhang: Massachusetts Institute of Technology
Albinas Cepauskas: Université Libre de Bruxelles (ULB)
Anastasiia Nadieina: Université Libre de Bruxelles (ULB)
Aurelien Thureau: Université Libre de Bruxelles (ULB)
Kyo Coppieters ‘t Wallant: Synchrotron SOLEIL
Chloé Martens: Synchrotron SOLEIL
Daniel C. Lim: Massachusetts Institute of Technology
Abel Garcia-Pino: Université Libre de Bruxelles (ULB)
Michael T. Laub: Massachusetts Institute of Technology

Nature, 2024, vol. 635, issue 8039, 728-735

Abstract: Abstract Eukaryotic innate immune systems use pattern recognition receptors to sense infection by detecting pathogen-associated molecular patterns, which then triggers an immune response. Bacteria have similarly evolved immunity proteins that sense certain components of their viral predators, known as bacteriophages1–6. Although different immunity proteins can recognize different phage-encoded triggers, individual bacterial immunity proteins have been found to sense only a single trigger during infection, suggesting a one-to-one relationship between bacterial pattern recognition receptors and their ligands7–11. Here we demonstrate that the antiphage defence protein CapRelSJ46 in Escherichia coli can directly bind and sense two completely unrelated and structurally different proteins using the same sensory domain, with overlapping but distinct interfaces. Our results highlight the notable versatility of an immune sensory domain, which may be a common property of antiphage defence systems that enables them to keep pace with their rapidly evolving viral predators. We found that Bas11 phages harbour both trigger proteins that are sensed by CapRelSJ46 during infection, and we demonstrate that such phages can fully evade CapRelSJ46 defence only when both triggers are mutated. Our work shows how a bacterial immune system that senses more than one trigger can help prevent phages from easily escaping detection, and it may allow the detection of a broader range of phages. More generally, our findings illustrate unexpected multifactorial sensing by bacterial defence systems and complex coevolutionary relationships between them and their phage-encoded triggers.

Date: 2024
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DOI: 10.1038/s41586-024-08039-y

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