 Inference of Relationships in Population Data Using Identity-by-Descent and Identity-by-StateEric L Stevens, Greg Heckenberg, Elisha D O Roberson, Joseph D Baugher, Thomas J Downey and Jonathan Pevsner PLOS Genetics, 2011, vol. 7, issue 9, 1-15 Abstract: It is an assumption of large, population-based datasets that samples are annotated accurately whether they correspond to known relationships or unrelated individuals. These annotations are key for a broad range of genetics applications. While many methods are available to assess relatedness that involve estimates of identity-by-descent (IBD) and/or identity-by-state (IBS) allele-sharing proportions, we developed a novel approach that estimates IBD0, 1, and 2 based on observed IBS within windows. When combined with genome-wide IBS information, it provides an intuitive and practical graphical approach with the capacity to analyze datasets with thousands of samples without prior information about relatedness between individuals or haplotypes. We applied the method to a commonly used Human Variation Panel consisting of 400 nominally unrelated individuals. Surprisingly, we identified identical, parent-child, and full-sibling relationships and reconstructed pedigrees. In two instances non-sibling pairs of individuals in these pedigrees had unexpected IBD2 levels, as well as multiple regions of homozygosity, implying inbreeding. This combined method allowed us to distinguish related individuals from those having atypical heterozygosity rates and determine which individuals were outliers with respect to their designated population. Additionally, it becomes increasingly difficult to identify distant relatedness using genome-wide IBS methods alone. However, our IBD method further identified distant relatedness between individuals within populations, supported by the presence of megabase-scale regions lacking IBS0 across individual chromosomes. We benchmarked our approach against the hidden Markov model of a leading software package (PLINK), showing improved calling of distantly related individuals, and we validated it using a known pedigree from a clinical study. The application of this approach could improve genome-wide association, linkage, heterozygosity, and other population genomics studies that rely on SNP genotype data. Author Summary: High-density microarrays measuring single nucleotide polymorphisms (SNPs) provide information about the genotypes across many loci. SNP genotypes observed for any two individuals can be compared in terms of identity-by-state (IBS), in which two individuals are observed to have 0, 1, or 2 alleles in common at a given locus, across a chromosomal region, or throughout the genome. These alleles may be shared identical-by-descent (IBD) in which 0, 1, or 2 alleles are inherited from a recent common ancestor, or they may be identical by chance because the allele is frequent in the population. The expected proportion of genome sharing between two individuals varies as a function of their genetic relatedness. We introduce a method to estimate IBD that can be used to analyze relatedness in pedigrees or in large-scale population studies with thousands of individuals. This can be combined with observed IBS to distinguish a variety of types of relatedness, providing theoretically justified results that are graphed in a manner that is straightforward to interpret. The methods we introduce are relevant to a variety of SNP applications including linkage and association studies and population genomics studies. Date: 2011 Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:plo:pgen00:1002287 DOI: 10.1371/journal.pgen.1002287 Access Statistics for this article More articles in PLOS Genetics from Public Library of Science |
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