TNF-α exacerbates SARS-CoV-2 infection by stimulating CXCL1 production from macrophages

Moe Kobayashi, Nene Kobayashi, Kyoka Deguchi, Seira Omori, Minami Nagai, Ryutaro Fukui, Isaiah Song, Shinji Fukuda, Kensuke Miyake and Takeshi Ichinohe

PLOS Pathogens, 2024, vol. 20, issue 12, 1-22

Abstract: Since most genetically modified mice are C57BL/6 background, a mouse-adapted SARS-CoV-2 that causes lethal infection in young C57BL/6 mice is useful for studying innate immune protection against SARS-CoV-2 infection. Here, we established two mouse-adapted SARS-CoV-2, ancestral and Delta variants, by serial passaging 80 times in C57BL/6 mice. Although young C57BL/6 mice were resistant to infection with the mouse-adapted ancestral SARS-CoV-2, the mouse-adapted SARS-CoV-2 Delta variant caused lethal infection in young C57BL/6 mice. In contrast, MyD88 and IFNAR1 KO mice exhibited resistance to lethal infection with the mouse-adapted SARS-CoV-2 Delta variant. Treatment with recombinant IFN-α/β at the time of infection protected mice from lethal infection with the mouse-adapted SARS-CoV-2 Delta variant, but intranasal administration of recombinant IFN-α/β at 2 days post infection exacerbated the disease severity following the mouse-adapted ancestral SARS-CoV-2 infection. Moreover, we showed that TNF-α amplified by type I IFN signals exacerbated the SARS-CoV-2 infection by stimulating CXCL1 production from macrophages and neutrophil recruitment into the lung tissue. Finally, we showed that intravenous administration to mice or hamsters with TNF protease inhibitor 2 alleviated the severity of SARS-CoV-2 and influenza virus infection. Our results uncover an unexpected mechanism by which type I interferon-mediated TNF-α signaling exacerbates the disease severity and will aid in the development of novel therapeutic strategies to treat respiratory virus infection and associated diseases such as influenza and COVID-19.Author summary: Coronavirus disease 2019 (COVID-19) cause severe morbidity and mortality worldwide. Although mounting evidence indicates that the virus-induced cytokine storm associates with severity of COVID-19, the pathological role of inflammatory cytokines in severe COVID-19 remains unknown. Here, we demonstrated that the virus-induced pulmonary TNF-α amplified by MyD88 and type I interferon receptor signals exacerbated the SARS-CoV-2 infection by stimulating CXCL1 production from macrophages. In addition, we found that TNF-α protease inhibitor 2 alleviated the severity of SARS-CoV-2 and influenza virus infection in mice and hamsters. Our results provide important insights into the role of type I interferon-mediated TNF-α signaling in neutrophil-induced lung pathology and will aid the development of novel therapeutic strategies to treat respiratory viral infections and associated diseases.

Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:plo:ppat00:1012776

DOI: 10.1371/journal.ppat.1012776

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