Location-dependent intracellular particle tracking using a cell-based coordinate system

Shada Abuhattoum and Daphne Weihs

Computer Methods in Biomechanics and Biomedical Engineering, 2013, vol. 16, issue 10, 1042-1049

Abstract: Intracellular structure and active processes have been studied by particle tracking using the motion of internalised probes. Intracellular particle motion is driven by a complex combination of active and thermal processes within heterogeneous and dynamically changing micro-environments. Regions in the cells may react differently to environmental changes or following treatment, exhibiting location-dependent responses. Hence, to reveal such responses, we introduce cell-specific polar coordinate systems. The coordinates are defined for each cell by its nucleus location and orientation, providing relative particle locations in the cytoplasm. The utility of our approach is demonstrated by comparing Adenosine Triphosphate (ATP)-depleted and control cells. In both cells, we observe differences in particle transport with the distance from the nucleus. Following ATP depletion, basic particle motion analysis shows an expected reduction in activity driving particle transport. However, it is our location-dependent approach which reveals that while morphology changes primarily at the cortex, the cell response is actually nearly uniform across the cytoplasm.

Date: 2013
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://hdl.handle.net/10.1080/10255842.2012.761694 (text/html)
Access to full text is restricted to subscribers.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:taf:gcmbxx:v:16:y:2013:i:10:p:1042-1049

Ordering information: This journal article can be ordered from
http://www.tandfonline.com/pricing/journal/gcmb20

DOI: 10.1080/10255842.2012.761694

Access Statistics for this article

Computer Methods in Biomechanics and Biomedical Engineering is currently edited by Director of Biomaterials John Middleton

More articles in Computer Methods in Biomechanics and Biomedical Engineering from Taylor & Francis Journals
Bibliographic data for series maintained by Chris Longhurst ().