Improving methanol production by Methylosinus trichosporium through the one factor at a time (OFAT) approach

Ahmad Razi Othman, Yap Jun Sheng, Noorhisham Tan Kofli and Siti Kartom Kamaruddin

Greenhouse Gases: Science and Technology, 2022, vol. 12, issue 5, 661-668

Abstract: The technology of methanol production from the fermentation of methanol‐producing bacteria (methanotroph) is still in the research phase. Maximizing the methanol production through bacteria fermentation in this process is a challenge to researchers. By optimizing the biological process, this technology can potentially fill the demand gap in methanol industries. Methylosinus trichosporium is a methanotroph that is used for the production of methanol in this analysis. Using OD600 analysis, the rate and growth profile for M. trichosporium is determined. The one factor at a time (OFAT) method was chosen to optimize the methanol production (percentage of methane, pH, and phosphate ion), and the optimum condition for this bacterium was determined. M. trichosporium showed a specific growth rate at 0.02 h–1 with the exponential phase reaching the maximum at 90 h of the cultivation period. Optimization analysis showed that the maximum methanol production by this bacterium was at a 1:1 methane–air ratio, a pH of 6.3–6.5, and 40 mM of phosphate ions. The ability of M. trichosporium to produce methanol was proved in this analysis with the maximum rate of production at 0.072 mg L–1 h–1. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.

Date: 2022
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