Transcriptome Sequencing of Agave angustifolia Reveals Conservation and Diversification in the Expression of Cinnamyl Alcohol Dehydrogenase Genes in Agave Species

Xing Huang, Bochao Xu, Shibei Tan, Yanlei Huang, Jingen Xi, Xu Qin, Tao Chen, Helong Chen, Xiaohan Yang and Kexian Yi
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Xing Huang: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Bochao Xu: School of Life Sciences, Hainan University, Haikou 570228, China
Shibei Tan: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Yanlei Huang: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Jingen Xi: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Xu Qin: Guangxi Subtropical Crops Research Institute, Nanning 530001, China
Tao Chen: Guangxi Subtropical Crops Research Institute, Nanning 530001, China
Helong Chen: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Xiaohan Yang: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
Kexian Yi: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Agriculture, 2022, vol. 12, issue 7, 1-10

Abstract: Agave angustifolia is an important crassulacean acid metabolism plant species, with wide applications in beverage and sisal fiber production. In this study, we carried out a transcriptome analysis of A . angustifolia leaves, generating a total of 58,482,436 clean reads through Illumina paired-end sequencing. De novo transcriptome assembly generated 67,314 unigenes, with about half of them having homologs in four public databases. In the Nr database, Asparagus officinalis was shown to be most closely related to agave, and the early angiosperm Amborella trichopoda was selected as an outgroup species. We further identified five, two, seven, seven, seven, six and six CAD genes in asparagus, amborella, A. deserti , A. tequilana , A. americana , A. hybrid H11648 and A. angustifolia , respectively. The maximum likelihood phylogenetic tree revealed the species-specific expansion of CAD genes in arabidopsis, rice and agave. The expression analysis indicated the conservatively expressed CAD1 / 2 / 4 / 6 , providing candidate targets for manipulation to improve lignin traits. The species-specific expression of CAD3 / 5 / 7 indicates the existence of different regulatory mechanisms controlling the expression of these genes in agave species. This study presents the first transcriptome dataset of A. angustifolia , facilitating future studies on lignin biosynthesis in agave.

Keywords: transcriptome; Agave angustifolia; cinnamyl alcohol dehydrogenase; phylogeny; gene expression (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2022
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