Friable Callus Induction of Hedera nepalensis var. sinensis

Dingfan Xu, Yanjun Liu, Yawen Liu, Junxuan Huang, Chunxia Wu and Jianke Li

Asian Agricultural Research, 2021, vol. 13, issue 02

Abstract: [Objectives] The purpose was to establish an induction system for friable callus of Hedera nepalensis var. sinensis with different parts. [Methods] By screening the most suitable explant and adjusting the hormone ratio of medium, friable calli of H. nepalensis var. sinensis were induced. [Results] The calli could be induced from leaves, petioles and stem segments, but the ideal explant was stem segments, with induction rate reaching 98%. The optimal medium for callus proliferation was MS + 0.5 mg/L KT + 1.0 mg/L 2,4-D + 30.0 g/L sucrose. After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose, favorable friable calli of H. nepalensis var. sinensis were obtained. [Conclusions] The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H. nepalensis var. sinensis.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2021
References: Add references at CitEc
Citations:

Downloads: (external link)
https://ageconsearch.umn.edu/record/316694/files/F ... 20sinensis%20%20.pdf (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:316694

DOI: 10.22004/ag.econ.316694

Access Statistics for this article

More articles in Asian Agricultural Research from USA-China Science and Culture Media Corporation
Bibliographic data for series maintained by AgEcon Search ().