Fluorescence-based PCR Detection of Goat Milk Powder Mixed with Cow milk

Yunxia Wang, Lijuan Jing, Zhijun Li, Cuizhi Li, Zhiyong Lu and Lijun Liu

Asian Agricultural Research, 2021, vol. 13, issue 08

Abstract: [Objectives] To amplify and identify the specific gene fragments of cattle and sheep by real-time fluorescence-based PCR, and quickly and accurately identify the adulteration problem of goat milk powder according to the differences of mitochondrial genes among different species. [Methods] The specificity, limit of detection and repeatability test were carried out by isolating somatic cells, extracting template DNA and determining the reaction conditions of real-time fluorescence-based PCR. [Results] The real-time fluorescence-based PCR method could be used to identify the bovine and ovine-derived materials of milk powder with high specificity, high sensitivity and good repeatability, and the limit of detection of milk components in goat milk powder could reach 0.01%. [Conclusions] The real-time fluorescence-based PCR method can be used to quickly and accurately detect the milk components in goat milk powder, and provide some technical support for the adulteration detection of dairy industry.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2021
References: Add references at CitEc
Citations:

Downloads: (external link)
https://ageconsearch.umn.edu/record/317691/files/F ... ith%20Cow%20milk.pdf (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:317691

DOI: 10.22004/ag.econ.317691

Access Statistics for this article

More articles in Asian Agricultural Research from USA-China Science and Culture Media Corporation
Bibliographic data for series maintained by AgEcon Search ().