Simultaneous Determination of Content of VK1 and VK2 in Milk and Dairy Products by High Performance Liquid Chromatography

Huili Gong, Guoxia Duan, Lijun Liu, Shuo Tang, Cuizhi Li and Zhiyong Lu

Asian Agricultural Research, 2022, vol. 14, issue 07

Abstract: [Objectives] To make simultaneous determination of vitamin K1 (VK1 ) and vitamin K2 (VK2, mainly MK-4, MK-7 and MK-9) in milk and dairy products. [Methods] A high performance liquid chromatographic method was developed for the simultaneous determination of vitamin K1 (VK1 ) and vitamin K2 (VK2, mainly MK-4, MK-7 and MK-9) in milk and dairy products. After enzymatic digestion, the samples were extracted with hexane for VK1, MK-4, MK-7 and MK-9, subjected to gradient elution at excitation wavelength 243 nm and emission wavelength 430 nm, detected by high performance liquid chromatography with fluorescence detector and quantified by external standard method. [Results] The linearity of VK1, MK-4, MK-7 and MK-9 was good in the concentration range of 2.5 -1000 ng/mL with the correlation coefficients greater than 0.999; The relative standard deviations (RSD) of VK1, MK-4, MK-7 and MK-9 in milk powder, liquid milk and yogurt were 1.32%-5.05%, 1.10%-2.48% and 2.20%-3.47%, respectively; the recovery rates of VK1, MK-4, MK-7 and MK-9 at different levels in milk powder, liquid milk and yogurt were 81.1%-108%, 81.8%-103% and 82.1%-99.2%, respectively. [Conclusions] The method is rapid, accurate, reproducible and capable of simultaneous determination of VK1, MK-4, MK-7 and MK-9.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:333812

DOI: 10.22004/ag.econ.333812

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