Proteomic analysis of Fusarium sp. NF01 revealed a multi-level regulatory machinery for lignite biodegradation

Xian Niu, Jianbin Zhang, Yonglu Suo and Jilagamazhi Fu

Energy, 2022, vol. 250, issue C

Abstract: Proteomic analysis provides vital bioinformation for revealing the mechanisms of coal biodegradation. Yet, proteomics-based studies of coal biodegradation are generally lacking. We first studied the physio-chemical changes in lignite biodegradation by a previously isolated fungus (Fusarium sp. NF01). The strain was able to degrade lignite showing significant changes in the ultraviolet–visible absorbance, surface structure and a 40.9% increased free radical concentration of residual lignite. Subsequently, isobaric tandem mass tags (TMT) and bioinformatic analysis were used for the quantitative and qualitative analyses of Fusarium sp. NF01 secretome. The expression levels of 62 proteins were significantly altered in the lignite-supplemented medium: 20 proteins were upregulated (fold-change, FC ≥ 2; P-value ≤0.05) and 42 proteins were downregulated (FC ≤ 0.5, P-value ≤0.05). Bioinformatic analysis indicated that Fusarium sp. NF01 acclimatized to the lignite-rich environment by regulating the expression of proteins involved in defense, lignite degradation, detoxification, and cellular metabolism. These findings may help promote sustainable and value-added coal utilization.

Keywords: Coal biodegradation; Fusarium; Lignite; Proteomics; Tandem mass tags (search for similar items in EconPapers)
Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0360544222006661
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:250:y:2022:i:c:s0360544222006661

DOI: 10.1016/j.energy.2022.123763

Access Statistics for this article

Energy is currently edited by Henrik Lund and Mark J. Kaiser

More articles in Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().