High biodesulfurization efficiency of oil by aerobic (Burkholderia sp. and Serratia sp.) and anaerobic bacteria using various additives

Marinos Stylianou, Charis G. Samanides, Ioannis Vyrides and Agapios Agapiou

Energy, 2023, vol. 282, issue C

Abstract: Eleven oil-polluted environmental samples were collected from different contaminated sites in Cyprus. The solid and liquid samples were enriched after exposure to dibenzothiophene (DBT) or 2-hydroxybiphenyl (2-HBP) as the sole sulfur and carbon sources. The aerobic bacteria were isolated and tested for their ability to biodesulfurize (BDS) oil. After the growth of isolates and DNA extraction (PCR amplification), the results revealed two prominent bacteria, Serratia sp. and Burkholderia sp., which were able to decrease DBT concentrations by up to 90%. Furthermore, it was identified that adding zeolite as a carrier in the respective solution increased the removal efficiency of DBT; Burkholderia sp. was the most dominant species and suppressed Stenotrophomonas sp. Then, the selected bacteria were tested in real oil samples (heating oil) and managed to remove 70–80% of the sulfur content. Anaerobic BDS using various anaerobic inocula was also tested as a proof of concept. Specifically, anaerobic inocula from digester sludge, drill cuttings, and secondary sludge showed around 60% DBT removal after ten days. Anaerolineaceae, Clostridium sensu stricto 12, and Rummeliibacillus were identified in those inocula in relatively high abundance. The addition of ZVI to anaerobic inocula resulted in a further increase in DBT removal.

Keywords: Carrier; Dibenzothiophene; DNA extraction; Enrichment; Sulfur; Pollution (search for similar items in EconPapers)
Date: 2023
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0360544223023447
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:eee:energy:v:282:y:2023:i:c:s0360544223023447

DOI: 10.1016/j.energy.2023.128950

Access Statistics for this article

Energy is currently edited by Henrik Lund and Mark J. Kaiser

More articles in Energy from Elsevier
Bibliographic data for series maintained by Catherine Liu ().