Molecular Characterization of Three Chemosensory Proteins from Carposina sasakii

Liu Liu, Guisheng Qiu, Huaijiang Zhang, Qiang Yue, Wentao Yan and Lina Sun ()
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Liu Liu: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China
Guisheng Qiu: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China
Huaijiang Zhang: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China
Qiang Yue: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China
Wentao Yan: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China
Lina Sun: Research Institute of Pomology, Chinese Academy of Agricultural Sciences, 98 South Street, Xinghai, Huludao 125100, China

Agriculture, 2023, vol. 13, issue 11, 1-17

Abstract: The peach fruit moth, Carposina sasakii , is part of the Carposinidae, and is harmful to the families Rosaceae and Rhamnaceae. C. sasakii lays eggs on the hairy surface of the fruit’s stalk cavity and calyx end. After hatching, the moth can bore into the fruits and feed on the flesh inside. Chemosensory proteins (CSPs) are a class of low-molecular-weight soluble carrier proteins that are highly evolutionarily conserved. To enhance our understanding of the recognition of host plant volatiles by CSPs of C. sasakii , the expression patterns and binding characteristics of CsasCSP7, CsasCSP9 and CsasCSP11 in C. sasakii were investigated. In our study, the results of real-time quantitative polymerase chain reaction (qPCR) assays demonstrate that CsasCSP7 and CsasCSP9 transcripts were abundantly expressed in the antennae of males, and CsasCSP11 was highly expressed in the wings of females. Fluorescence competitive binding assays with 38 candidate ligands showed that CsasCSP7 could bind to benzaldehyde and dodecanal, whereas CsasCSP9 bound to butyl octanoate, decanal and (-)-beta-pinene. CsasCSP11 could also bind to1-hexanol, beta-ocimene and 6-methyl-5-hepten-2-one. Our results suggest that CsasCSP7, CsasCSP9 and CsasCSP11 may play a crucial role in locating the host plant of C. sasakii .

Keywords: Carposina sasakii; chemosensory proteins; fluorescence competitive binding; plant volatile organic compounds (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
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