Genome Survey of Stipa breviflora Griseb. Using Next-Generation Sequencing

Xiangjun Yun, Jinrui Wu, Bo Xu, Shijie Lv, Le Zhang, Wenguang Zhang, Shixian Sun, Guixiang Liu (), Yazhou Zu and Bin Liu
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Xiangjun Yun: Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
Jinrui Wu: Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
Bo Xu: Inner Mongolia Forestry Monitoring and Planning Institute, Hohhot 010020, China
Shijie Lv: College of Science, Inner Mongolia Agricultural University, Hohhot 010018, China
Le Zhang: Inner Mongolia Forestry Monitoring and Planning Institute, Hohhot 010020, China
Wenguang Zhang: College of Science, Inner Mongolia Agricultural University, Hohhot 010018, China
Shixian Sun: Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
Guixiang Liu: Grassland Research Institute, Chinese Academy of Agriculture Sciences, Hohhot 010019, China
Yazhou Zu: Nei Mongol BioNew Technology, Hohhot 010018, China
Bin Liu: Nei Mongol BioNew Technology, Hohhot 010018, China

Agriculture, 2023, vol. 13, issue 12, 1-16

Abstract: Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora , a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. To the best of our knowledge, the present study is the first to sequence, assemble, and annotate the S. breviflora genome. In total, 2,781,544 contigs were assembled, and 2,600,873 scaffolds were obtained, resulting in a total length of 649,849,683 bp. The number of scaffolds greater than 1 kb was 70,770. We annotated the assembled genome (>121 kb), conducted a selective sweep analysis, and ultimately succeeded in assembling the Matk gene of S. breviflora . More importantly, our research identified 26 scaffolds that may be responsible for the drought tolerance of S. breviflora Griseb. In summary, the data obtained regarding S. breviflora will be of great significance for future research.

Keywords: gene annotation; genome survey; next-generation sequencing; selective sweep analysis; Stipa breviflora Griseb (search for similar items in EconPapers)
JEL-codes: Q1 Q10 Q11 Q12 Q13 Q14 Q15 Q16 Q17 Q18 (search for similar items in EconPapers)
Date: 2023
References: View references in EconPapers View complete reference list from CitEc
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