Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

Pierre Gönczy (), Christophe Echeverri, Karen Oegema, Alan Coulson, Steven J. M. Jones, Richard R. Copley, John Duperon, Jeff Oegema, Michael Brehm, Etienne Cassin, Eva Hannak, Matthew Kirkham, Silke Pichler, Kathrin Flohrs, Anoesjka Goessen, Sebastian Leidel, Anne-Marie Alleaume, Cécilie Martin, Nurhan Özlü, Peer Bork and Anthony A. Hyman ()
Additional contact information
Pierre Gönczy: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Christophe Echeverri: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Karen Oegema: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Alan Coulson: The Sanger Centre, Wellcome Trust Genome Campus
Steven J. M. Jones: Genome Sequence Centre, British Columbia Cancer Research Centre
Richard R. Copley: European Molecular Biology Laboratory (EMBL)
John Duperon: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Jeff Oegema: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Michael Brehm: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Etienne Cassin: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Eva Hannak: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Matthew Kirkham: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Silke Pichler: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Kathrin Flohrs: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Anoesjka Goessen: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Sebastian Leidel: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Anne-Marie Alleaume: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Cécilie Martin: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Nurhan Özlü: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)
Peer Bork: European Molecular Biology Laboratory (EMBL)
Anthony A. Hyman: Max-Planck-Institute for Cell Biology and Genetics (MPI-CBG)

Nature, 2000, vol. 408, issue 6810, 331-336

Abstract: Abstract Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of ∼96% of the ∼2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (∼6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that ∼47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.

Date: 2000
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DOI: 10.1038/35042526

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