Co-translational mRNA decay in Saccharomyces cerevisiae

Wenqian Hu, Thomas J. Sweet, Sangpen Chamnongpol, Kristian E. Baker and Jeff Coller ()
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Wenqian Hu: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
Thomas J. Sweet: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
Sangpen Chamnongpol: Affymetrix Inc., 26111 Miles Road, Cleveland, Ohio 44128, USA
Kristian E. Baker: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
Jeff Coller: Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA

Nature, 2009, vol. 461, issue 7261, 225-229

Abstract: Abstract The rates of RNA decay and transcription determine the steady-state levels of all messenger RNA and both can be subject to regulation. Although the details of transcriptional regulation are becoming increasingly understood, the mechanism(s) controlling mRNA decay remain unclear. In yeast, a major pathway of mRNA decay begins with deadenylation followed by decapping and 5′–3′ exonuclease digestion. Importantly, it is hypothesized that ribosomes must be removed from mRNA before transcripts are destroyed. Contrary to this prediction, here we show that decay takes place while mRNAs are associated with actively translating ribosomes. The data indicate that dissociation of ribosomes from mRNA is not a prerequisite for decay and we suggest that the 5′–3′ polarity of mRNA degradation has evolved to ensure that the last translocating ribosome can complete translation.

Date: 2009
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DOI: 10.1038/nature08265

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