GBStools: A Statistical Method for Estimating Allelic Dropout in Reduced Representation Sequencing Data

Thomas F Cooke, Muh-Ching Yee, Marina Muzzio, Alexandra Sockell, Ryan Bell, Omar E Cornejo, Joanna L Kelley, Graciela Bailliet, Claudio M Bravi, Carlos D Bustamante and Eimear E Kenny

PLOS Genetics, 2016, vol. 12, issue 2, 1-18

Abstract: Reduced representation sequencing methods such as genotyping-by-sequencing (GBS) enable low-cost measurement of genetic variation without the need for a reference genome assembly. These methods are widely used in genetic mapping and population genetics studies, especially with non-model organisms. Variant calling error rates, however, are higher in GBS than in standard sequencing, in particular due to restriction site polymorphisms, and few computational tools exist that specifically model and correct these errors. We developed a statistical method to remove errors caused by restriction site polymorphisms, implemented in the software package GBStools. We evaluated it in several simulated data sets, varying in number of samples, mean coverage and population mutation rate, and in two empirical human data sets (N = 8 and N = 63 samples). In our simulations, GBStools improved genotype accuracy more than commonly used filters such as Hardy-Weinberg equilibrium p-values. GBStools is most effective at removing genotype errors in data sets over 100 samples when coverage is 40X or higher, and the improvement is most pronounced in species with high genomic diversity. We also demonstrate the utility of GBS and GBStools for human population genetic inference in Argentine populations and reveal widely varying individual ancestry proportions and an excess of singletons, consistent with recent population growth.Author Summary: Eukaryotic genomes range from millions to billions of base pairs in size, but for many genetic experiments it is sufficient to gather information from just a fraction of these sites. In practice, selecting a consistent set of sites can be achieved by cutting genomic DNA with enzymes that recognize DNA sequence motifs, and then sequencing the ends of the resulting fragments. The advantages of this well-known approach are its low cost relative to whole-genome sequencing (WGS) and that it does not require a sequenced genome. These methods, for example genotyping-by-sequencing (GBS), are popular for mapping genes and studying population genetics, particularly in non-model organisms. Here we demonstrate, however, that computational tools designed for WGS are insufficient for handling certain error types that arise in GBS and other similar methods. We present a modified protocol for GBS and a statistical method for detecting these errors, implemented in the software package GBStools. We tested our methods on human DNA samples from Argentine populations. Our results reveal widely varying degrees of European and Native American ancestry, and that rare genetic variants are more numerous than would be expected in a population with constant size.

Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005631 (text/html)
https://journals.plos.org/plosgenetics/article/fil ... 05631&type=printable (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:plo:pgen00:1005631

DOI: 10.1371/journal.pgen.1005631

Access Statistics for this article

More articles in PLOS Genetics from Public Library of Science
Bibliographic data for series maintained by plosgenetics ().