Full atomistic model of prion structure and conversion

Giovanni Spagnolli, Marta Rigoli, Simone Orioli, Alejandro M Sevillano, Pietro Faccioli, Holger Wille, Emiliano Biasini and Jesús R Requena

PLOS Pathogens, 2019, vol. 15, issue 7, 1-18

Abstract: Prions are unusual protein assemblies that propagate their conformationally-encoded information in absence of nucleic acids. The first prion identified, the scrapie isoform (PrPSc) of the cellular prion protein (PrPC), caused epidemic and epizootic episodes [1]. Most aggregates of other misfolding-prone proteins are amyloids, often arranged in a Parallel-In-Register-β-Sheet (PIRIBS) [2] or β-solenoid conformations [3]. Similar folding models have also been proposed for PrPSc, although none of these have been confirmed experimentally. Recent cryo-electron microscopy (cryo-EM) and X-ray fiber-diffraction studies provided evidence that PrPSc is structured as a 4-rung β-solenoid (4RβS) [4, 5]. Here, we combined different experimental data and computational techniques to build the first physically-plausible, atomic resolution model of mouse PrPSc, based on the 4RβS architecture. The stability of this new PrPSc model, as assessed by Molecular Dynamics (MD) simulations, was found to be comparable to that of the prion forming domain of Het-s, a naturally-occurring β-solenoid. Importantly, the 4RβS arrangement allowed the first simulation of the sequence of events underlying PrPC conversion into PrPSc. This study provides the most updated, experimentally-driven and physically-coherent model of PrPSc, together with an unprecedented reconstruction of the mechanism underlying the self-catalytic propagation of prions.Author summary: Prions are unusual infectious pathogens that do not contain any nucleic acid. They consist of assemblies of misfolded proteins. The scrapie isoform of the mammalian prion protein, PrPSc, is the most notorious prion, and is responsible for deadly neurodegenerative diseases affecting humans, like Creutzfeldt-Jakob disease, and animals, such as bovine spongiform encephalopathy (“mad cow disease”) and chronic wasting disease affecting elk and deer in North America and, more recently, Europe). Understanding the structure (“shape”) of the PrPSc prion is critical to understand how it propagates. We have created a very detailed model of PrPSc, which includes all its atoms, using computational techniques. The model resembles a 4-rung cork-screw. The model is stable and agrees with all available experimental data on PrPSc. This structure allowed us to model for the first time the process of prion propagation at high resolution. These data inspire several new hypotheses to elucidate prion biology and design therapeutics for prion diseases

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:plo:ppat00:1007864

DOI: 10.1371/journal.ppat.1007864

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